Quantification of replication factory size and number. Three dimensional volume renderings of the restored Z stacks were produced in SVI's Huygens imaging software (panel A - images shown are using a garbage volume of 5 voxels). Scale bars = 2 μm. Colours represent increasing intensities from blue to red. The number of objects in each cell was then counted using a selection of different garbage volume thresholds. The average number of objects per μm3 at each garbage volume (as indicated on the right) is presented (B). The total number of objects for each threshold corresponds to the top of the appropriately shaded bar in each category. These data are also presented in table 1. The same software was used to determine the maximum axial width of an object in each category at garbage volume 5 (C). Error bars represent average deviations from the mean (n = 3-5).
Cseresnyes et al. BMC Cell Biology 2009 10:88 doi:10.1186/1471-2121-10-88