STED imaging of replication factories. MRC5 cells were labelled with ATTO 647-linked secondary antibodies and anti-RPA (in A) or anti-PCNA (in B) primary antibodies. Images were acquired sequentially, in normal confocal mode (green) then using the STED setup (magenta). The lower panels are magnified regions of the cells as indicated. Scale bars = 2 μm.
Cseresnyes et al. BMC Cell Biology 2009 10:88 doi:10.1186/1471-2121-10-88