RPA and PCNA labelled replication factories. A and B: MRC5 cells were pulse labelled for 10 minutes with 40 μM EdU to label newly synthesised DNA which was then visualised in the green channel alongside indirect immunofluorescence (in magenta) from anti-PCNA or -RPA antibodies respectively. C and D: cells were co-labelled with anti-RPA monoclonal and anti-PCNA polyclonal antibodies in green and magenta (RPA-green in C and PCNA-green in D). Scale bars = 2 μm.
Cseresnyes et al. BMC Cell Biology 2009 10:88 doi:10.1186/1471-2121-10-88