Functional mitochondrial analysis of Kloch1-1 cells transformed with CpKlCMD1 plasmid. (A) Estimation of ROS accumulation in WT cells (black bar) and Kloch1-1 mutant strain harbouring empty (dark grey bar) or CpKlCMD1 (light grey bar) vectors by DHR-staining after growth to exponential phase in SD medium. The values were the mean of three independent experiments and showed an SD < 10%. (B) DASPMI staining of mutant cells transformed with centromeric (left side of the panel) or multicopy (right side of the panel) plasmids containing KlCMD1 gene.
Zanni et al. BMC Cell Biology 2009 10:86 doi:10.1186/1471-2121-10-86