Oxidative stress in Kloch1-1 cells. (A) Estimation of ROS accumulation in the indicated strains by DHR-staining after growth to exponential phase in SD medium. Measurements were also obtained after exposure to H2O2 for 2 h. (B) Cell viability after H2O2 exposure: parental strain (black bars) and cells lacking α1,6-mannosyltransferase activity (white bars), grown to exponential phase on YPD medium, were challenged with 20 mM H2O2 for 2 or 5 h. The viability was evaluated plating the samples on YPD and was expressed as the CFU percentage of the corresponding untreated cultures. The values of both panels were the mean of three independent experiments and showed an SD < 10%.
Zanni et al. BMC Cell Biology 2009 10:86 doi:10.1186/1471-2121-10-86