Interaction of CLN5 with the α- and β-subunits of the ATP synthase. GST-hPPT1 and GST-mCLN5 fusion proteins were used to pull down interacting proteins from enriched lysosomal/mitochondrial fraction extracted from the liver of wild type Cln1-/- and Cln5 -/- mice. The bound proteins were analyzed by Western blot using monoclonal antibodies for α- and β-ATP synthase. Load = 10 μg of total protein from mouse liver extract.
Lyly et al. BMC Cell Biology 2009 10:83 doi:10.1186/1471-2121-10-83