Table 1

Biochemical Binding Parameters from Solid Phase Assays

actin

Bmax (μM)

Kapp (μM)

R


α-G-

2.10 ± 0.07

0.031 ± 0.007

0.99

α-F-

2.31 ± 0.05

0.030 ± 0.004

0.99

β/γ-G-

1.90 ± 0.06

0.029 ± 0.005

0.99

β/γ-F-

2.01 ± 0.04

0.027 ± 0.003

0.99


Conditions were the same as those referred to in Figure 1. Binding parameters were estimated with the non-linear curve fit Hyperbl function (ΔA450 = Bmax * [tau]/(Kapp+ [tau]) in the Origin program.

He et al. BMC Cell Biology 2009 10:81   doi:10.1186/1471-2121-10-81

Open Data