Solid phase assay studies on the interaction of tauPRD with F-actin. 50 μl skeletal muscle F-actin (5 μg/ml) was added to solid phase assay wells. Aliquots of different concentrations of tauPRD (○), tau (□) and tauMTBD (△) were incubated with actin in binding buffer. Immunoreactivity was monitored using secondary antibody-labelled HRP. Each point represents the mean of five determinations. Binding parameters were estimated by fitting data using the Hyperbl function in Origin (Table 2).
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81