Low speed co-sedimentation assay of G-actin in the presence of tau and tau mutants. Tau and tau mutants were incubated with G-actin in a 1:1 mass ratio at 37°C in binding buffer and then centrifuged (25,000 g, 4°C, 30 min). Before electrophoresis on gels, pellets were resuspended with 20 mM Tris-HCl (pH 8.0) and boiled for 5 min. Rabbit skeletal muscle G-actin (panel a) and human platelet G-actin (panel b) were used. Actin alone was used as negative control. Different concentrations of tauΔPRD&MTBD reacted with G-actin. The pellets were electrophoresed on 12% SDS-PAGE gels after the centrifugation (panel c).
He et al. BMC Cell Biology 2009 10:81 doi:10.1186/1471-2121-10-81