Research article
RNAi mediated acute depletion of Retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation
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* Corresponding author: Aldo Di Leonardo adileon@unipa.it
1 Dipartimento di Biologia Cellulare e dello Sviluppo "A. Monroy", Università di Palermo, viale delle Scienze, Palermo, Italy
2 Dipartimento di Discipline Chirurgiche e Oncologiche, Laboratorio di Patofisiologia Cellulare e Molecolare Università di Palermo, Palermo, Italy
BMC Cell Biology 2009, 10:79 doi:10.1186/1471-2121-10-79
Published: 2 November 2009Additional files
Additional file 1:
FACScan analysis of colcemid treated human fibroblasts. Flow citometry analysis showed increase in G2/M of the cells treated with colcemid up to 16 hours. Cell cycle distribution was determined by using FACScanto (Becton Dickinson) and analyzed by ModFit software (Verity).
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