Table 1

Changes in [Ca2+]i in response to CCK-8 alone and in the presence of ethanol, and effect of alcohol dehydrogenase inhibition by 4-MP and cinnamtannin B-1

CCK-8

CCK-8/EtOH

CCK-8/EtOH/4-MP

CCK-8/EtOH/CinB


Peak [Ca2+]i (nM)

314.30 ± 17.39 n = 17

462.10 ± 22.26 n = 14

***

469.40 ± 15.30 n = 7

***

448.00 ± 19.99 n = 11

*


Steady level (nM)

136.30 ± 7.75 n = 17

173.10 ± 9.76 n = 14

**

140.10 ± 9.64 n = 7

134.40 ± 11.21 n = 11

††


Rate of decay

(Δnmol/s)

0.0093 ± 0.0014 n = 17

0.0051 ± 0.0004 n = 14

*

0.0105 ± 0.0014 n = 7

††

0.0077 ± 0.0006 n = 11

††


Total Ca2+ (nM)

27930 ± 2801 n = 17

43380 ± 4844 n = 14

**

37470 ± 2010 n = 7

*

39610 ± 5163 n = 11

*


Pancreatic acinar cells were stimulated with 1 nM CCK-8 alone, in the presence of 50 mM ethanol (EtOH), after preincubation in the presence of 1 mM of the alcohol dehydrogenase inhibitor 4-MP or following preincubation in the presence of 10 μM of the antioxidant cinnamtannin B-1 (CinB). Following CCK-8 stimulation, the peak [Ca2+]i response, the "steady-state level" achieved five minutes after application of the stimulus, the total Ca2+ mobilization and the rate of decay of [Ca2+]i towards basal values were calculated (*, P < 0.05; **, P < 0.01; ***, P < 0.001 vs CCK-8; †, P < 0.05; ††, P < 0.01 vs CCK-8 plus EtOH).

Fernández-Sánchez et al. BMC Cell Biology 2009 10:77   doi:10.1186/1471-2121-10-77

Open Data