Changes in [Ca2+]i in response to CCK-8 and effect of ethanol and cinnamtannin B-1. Time-course of changes in [Ca2+]i in fura-2-loaded mouse pancreatic acinar cells. Cells were stimulated with 1 nM CCK-8 in the presence of 50 mM ethanol (EtOH), added 1 minute prior to CCK-8, and after 30 minutes preincubation in the presence of 10 μM cinnamtannin B-1 (full circles), or only in the presence of 50 mM ethanol (open circles). Experiments were carried out in the presence of Ca2+ in the extracellular medium. The arrow indicates the time point at which CCK-8 was added to the cells. The traces are typical of 11-14 such independent experiments.
Fernández-Sánchez et al. BMC Cell Biology 2009 10:77 doi:10.1186/1471-2121-10-77