The binding of importin β to Tpr in the solid phase assay is sensitive to RanGTP. The binding of 100 nM importin β to Tpr adsorbed to microtiter wells was analyzed in the absence, or in the presence of 0.1, 0.2, 0.5 and 1 μM RanGMP-PNP or RanGDPβS, as indicated. The bound importin β was quantified as described in materials and methods.
Ben-Efraim et al. BMC Cell Biology 2009 10:74 doi:10.1186/1471-2121-10-74