Table 1 |
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Effect of Chlorate on [35S]-SO4 Incorporation into Proteoglycans and Glycosaminoglycans in C2C12 Myoblasts. |
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cpm/mg DNA |
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Control |
Chlorate |
|
|
Medium |
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Proteoglycans |
3950 ± 520 |
240 ± 40 |
|
Glycosaminoglycans |
1990 ± 570 |
520 ± 110 |
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Extracts |
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Proteoglycans |
11180 ± 550 |
820 ± 20 |
|
Glycosaminoglycans |
3820 ± 460 |
290 ± 100 |
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|
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C2C12 cells were incubated for 48 hours with 30 mM sodium chlorate. Sulfate incorporation during the last 18 hours was measured in medium and detergent extracts. [35S]Sulfate radioactivity for total (proteoglycans plus glycosaminoglycans) and trichloroacetic acid-precipitated material (proteoglycans) samples, spotted on filters containing the cationic detergent cetyl pyridinium chloride (CPC), was measured with liquid scintillation counting. DNA content was measured in same aliquots of detergent extracts (13.3 μg/μl for control and 18.0 μg/μl for chlorate treated cells). The specific activity values correspond to the means ± SD obtained from three different plates. |
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Osses et al. BMC Cell Biology 2009 10:73 doi:10.1186/1471-2121-10-73 |
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