Table 1

Effect of Chlorate on [35S]-SO4 Incorporation into Proteoglycans and Glycosaminoglycans in C2C12 Myoblasts.

cpm/mg DNA


Control

Chlorate

Medium

Proteoglycans

3950 ± 520

240 ± 40

Glycosaminoglycans

1990 ± 570

520 ± 110

Extracts

Proteoglycans

11180 ± 550

820 ± 20

Glycosaminoglycans

3820 ± 460

290 ± 100


C2C12 cells were incubated for 48 hours with 30 mM sodium chlorate. Sulfate incorporation during the last 18 hours was measured in medium and detergent extracts. [35S]Sulfate radioactivity for total (proteoglycans plus glycosaminoglycans) and trichloroacetic acid-precipitated material (proteoglycans) samples, spotted on filters containing the cationic detergent cetyl pyridinium chloride (CPC), was measured with liquid scintillation counting. DNA content was measured in same aliquots of detergent extracts (13.3 μg/μl for control and 18.0 μg/μl for chlorate treated cells). The specific activity values correspond to the means ± SD obtained from three different plates.

Osses et al. BMC Cell Biology 2009 10:73   doi:10.1186/1471-2121-10-73

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