Chondroitin sulfate proteoglycans reduce cell surface area in Oli-neu cells. (A) Oli-neu cells were cultured for 12 h on coverslips coated with different concentrations of chondroitin sulfate proteoglycans (CSPG), stained with Alexa Fluor 488 conjugated wheat germ agglutinin (WGA) and analyzed for changes in cell surface area. Scale bar, 10 μm. (B) Quantification of the surface area of Oli-neu cells cultured on CSPG coated coverslips with or without 10 μM Y27632, C3 transferase or (C) 50 μM blebbistatin (means ± SEM; n > 80 cells, *p < 0,05, **p < 0,01, ***p < 0,001).
Kippert et al. BMC Cell Biology 2009 10:71 doi:10.1186/1471-2121-10-71