Integrins in cell surface expansion and endocytosis in Oli-neu cells. (A) Oli-neu cells were treated for 8 h with 100 nM RGD-peptide, 100 nM inactive RAD-peptide, 10 μM Y27632 and 50 μM blebbistatin (blebb) as indicated. Cells were stained with Alexa Fluor 488 conjugated wheat germ agglutinin (green). Scale bar, 10 μm. (B) Changes in relative surface area were quantified by image analysis as described in Material and Methods. (C) Changes in relative surface area shown for cells grown on fibronectin. (D) Quantitative analysis of dextran uptake (30 min) in Oli-neu cells treated with 100 nM RGD-peptide or inactive RAD-peptide for 8 h. Values represent means ± SEM (n > 70 cells, **p < 0,01; ***p < 0,001).
Kippert et al. BMC Cell Biology 2009 10:71 doi:10.1186/1471-2121-10-71