(a) Schematic representation of N-terminally-deleted MAL2 proteins (defined in Table 1) employed in Y2H analyses. (b) Results of testing interactions between full-length and deleted MAL2 bait proteins and MUC1 (prey#1) prey protein in Hf7c cells using the Y2H system. (-) indicates no detectable growth of co-transformants on triple drop-out plates after 6–8 days incubation at 30°C while (++) indicates growth after 3–4 days. Results shown are representative of those obtained in 3 independent experiments.
Fanayan et al. BMC Cell Biology 2009 10:7 doi:10.1186/1471-2121-10-7