(a) Schematic diagram of MUC1 preys (prey#1-prey#7) isolated during Y2H screen. All prey proteins were predicted to include the SEA module, the transmembrane domain (TM) and the cytoplasmic tail (CT). The length of the extracellular tandem repeat is not shown to scale in full-length MUC1. (b) Deleting the MAL2 N-terminus does not affect its binding to MUC1 but abrogates its interaction with D52-like proteins. Interactions were tested between MAL, MAL2 or MAL2ΔN, and MUC1 (prey#1) or D52-like proteins in Hf7c cells using the Y2H system. (-) indicates no detectable growth of co-transformants on triple drop-out plates after 6–8 days at 30°C, (++) indicates growth after 3–4 days and (+++) indicates growth after 1–2 days. Identical results were obtained for all 3 D52-like baits, which were grouped together in a single column. Results shown are representative of those obtained in 3 independent experiments.
Fanayan et al. BMC Cell Biology 2009 10:7 doi:10.1186/1471-2121-10-7