Additional file 3.

Effect of NES addition on nuclear localized proteins. (A) We fused two assay-positive proteins (NANOG and ELK1 in Additional File 4) with the nuclear export sequence (NES) of protein kinase inhibitor alpha (PKIA) at the carboxy terminus and conducted the assay. The results were normalized with those obtained with native proteins. (B) GFP-fusion assay of NANOG and ELK1 with/without the NES. Nucleus was stained by DAPI.

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Hoat et al. BMC Cell Biology 2009 10:69   doi:10.1186/1471-2121-10-69