Additional file 6.
Localization of CRK9 in bloodstream form cells. The data shows that CRK9 localizes to the nuclei of bloodstream form cells. The pc-CRK9-3HA-BLA plasmid was linearized, transfected into 90-13 bloodstream form T. brucei cells, and stable cell lines were selected using blasticidin (10 μg/ml) and cloned to express CRK9-3HA at the endogenous level. Transfected and control cells were analyzed by Western blotting for the HA tag as indicated (A). The blot were stripped and probed with anti-α-tubulin antibody to verify equal sample loading. Localization of CRK9 was determined by fixing and staining the transfected cells with a FITC-conjugated anti-HA antibody (B). DAPI was used to visualize the nucleus and kinetoplast and merged images were created using the imageJ software.
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Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68