Additional file 5.
CRK9 RNAi in bloodstream form cells. The data presented here shows that CRK9 depletion has no effect on bloodstream form cells. The pZJM-CRK9 vector was transfected into 90-13 bloodstream form T. brucei cells, selected with phleomycin and followed by single cell cloning. RNAi was induced as previously described and cell number monitored daily. Real time quantitative RT-PCR was used to monitor the depletion of CRK9 after 2 days of RNAi (insets); with α-tubulin serving as the loading control.
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Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68