CRK9 interacts with cyclin B2. Recombinant GST-CRK9 was purified from transformed E. coli and incubated with the in vitro transcribed/translated HA-tagged cyclins E1 and B2, respectively. The GST protein complexes were bound to glutathione agarose, washed extensively, and analyzed by SDS PAGE followed by anti-HA Western blotting (top and central panels, as indicated) or total protein staining as loading controls (bottom panel). GST and glutathione beads without fused proteins attached served as negative controls.
Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68