Morphology of CRK9 depleted cells. The control cells (without RNAi induction) and the cells depleted of CRK9 by RNAi from the same set of experiments in Figure 1 were fixed and examined by light microscopy 1-5 days after induction of RNAi (as indicated in the top panel, 2A). The cells were classified as normal when the ratio of lengths between the major and minor axis was greater than 5:1but as morphologically rounded when the ratio was below 3:1. The frequency of cells appearing in normal and rounded morphology was tabulated from ~200 cells in each sample (bottom panel, 2B).
Gourguechon and Wang BMC Cell Biology 2009 10:68 doi:10.1186/1471-2121-10-68