Additional file 1.

FLIP analysis of GFP-RanQ69L. Images of a cell expressing nuclear GFP-RanQ69L and after successive cycles of photobleaching within a defined spot within the nucleus. The cell was subjected to 100 cycles of laser-bleaching, each of 20 ms duration. Image data were exported from softWoRx as MPEG files, from which frames were imported into iMovie and Quicktime software.

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Hutchins et al. BMC Cell Biology 2009 10:66   doi:10.1186/1471-2121-10-66