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Open Access Open Badges Research article

Dynamic localisation of Ran GTPase during the cell cycle

James RA Hutchins12, William J Moore13 and Paul R Clarke1*

Author Affiliations

1 Biomedical Research Institute, College of Medicine, Dentistry and Nursing, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, UK

2 Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria

3 Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK

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BMC Cell Biology 2009, 10:66  doi:10.1186/1471-2121-10-66

Published: 18 September 2009

Additional files

Additional file 1:

FLIP analysis of GFP-RanQ69L. Images of a cell expressing nuclear GFP-RanQ69L and after successive cycles of photobleaching within a defined spot within the nucleus. The cell was subjected to 100 cycles of laser-bleaching, each of 20 ms duration. Image data were exported from softWoRx as MPEG files, from which frames were imported into iMovie and Quicktime software.

Format: ZIP Size: 1.5MB Download file

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