Recovery studies. Colony formation studies of Ag-np treated IMR-90 and U251 cells: The cells after Ag-np exposure were allowed to recover from stress. The time in days taken for the cells to reach confluence is expressed as the recovery period (Y-axis) (A). Concentrations of nanoparticles are indicated on the X- axis. Cells treated with all concentrations except 400 μg/mL recovered. *p < 0.05. (B) Cell cycle analysis of recovered cells showing absence of cell cycle arrest (n = 3). (C) Untreated cancer cells showing recovery and (D) Ag-np treated (25 μg/mL) cells forming colonies (1 week). (E). Cells treated with higher concentration of Ag-np (200 μg/mL) did not form colonies. The morphology of the cells under recovery deteriorated with time. (F) Control cells with proper protoplasmic extensions. (G) Ag-np treated cancer cells showing unhealthy cells with no proper protoplasmic extensions (2 weeks). (H) Morphological deterioration with time suggesting the onset of cell death cascades.
AshaRani et al. BMC Cell Biology 2009 10:65 doi:10.1186/1471-2121-10-65