Open Access Research article

Inhibition of eIF2α dephosphorylation inhibits ErbB2-induced deregulation of mammary acinar morphogenesis

Sharon J Sequeira23, Huei Chi Wen12, Alvaro Avivar-Valderas1, Eduardo F Farias1 and Julio A Aguirre-Ghiso12*

Author Affiliations

1 Department of Medicine and Department of Otolaryngology, Division of Hematology and Oncology, Tisch Cancer Institute at Mount Sinai, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA

2 Department of Biomedical Sciences, School of Public Health and Gen*NY*sis Center for Excellence in Cancer Genomics, University at Albany, State University of New York, One Discovery Drive, Rensselaer, NY 12144-2346, USA

3 Department of Biological Sciences, Life Sciences Research Building 1113, University at Albany, State University of New York, 1400 Washington Ave, Albany, NY 12222, USA

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BMC Cell Biology 2009, 10:64  doi:10.1186/1471-2121-10-64

Published: 15 September 2009

Additional files

Additional file 1:

Supplementary figure 1. (A) Western blot for GADD34 from lysates of cells grown in monolayer conditions. GAPDH was used as loading control. (B) Graph showing viability of cells treated with vehicle or 10 μg/ml salubrinal in adhered conditions for the indicated time points, using Trypan blue exclusion. Points indicate average ± SD. (C) Graph showing the effect of salubrinal on 2D cellular proliferation. Cells were treated with vehicle or 10 μg/ml salubrinal in adhered conditions for the indicated time points before detachment and counting using a hemocytometer. Bars indicate average ± SD.

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