Effect of siRNA PAR-2.3 on PAR-2 expression in P815 cells. P815 cells were transfected with siRNA PAR-2.3 for 24, 48 and 72 h. The PAR-2 expression was determined by real-time quantitative PCR (A), flow cytometry (B), Western blot (C) and immunofluorescent analysis (D). In (C) and (D), 1 = 3 nM, 2 = 10 nM and 3 = 30 nM of siRNA PAR-2.3. Values shown are mean ± SEM for four to five independent experiments. * P < 0.05 compared with the untransfected control.
Qiao et al. BMC Cell Biology 2009 10:62 doi:10.1186/1471-2121-10-62