ERp72 and GRP78/BiP protein are elevated and ERp72 oxidized state is increased in AdDNPerk 832/13 beta cells. A. Triplicate samples were probed with antibodies to ERp72, ERp57, BiP and actin, which served as the loading control. B. ERp72 expression was increased in islets from P2 Perk KO mice. Samples are from duplicate mice for each genotype. C. At 24 hr or 36 hr post-transduction, protein samples were isolated and treated with AMS to differentiate the reduced and oxidized forms of ERp72 and ERp57 on PAGE gels. Western blots showed increased oxidized isoforms of both ERp72 and ERp57 in 832/13 cells infected with AdDNPerk compared to cells infected with AdLacZ.
Feng et al. BMC Cell Biology 2009 10:61 doi:10.1186/1471-2121-10-61