Basal levels of processed ATF6 are elevated in PERK-ablated cells. AD293 cells were co-transfected with EYFP-ATF6a and either empty vector or DNPerk. Each type of co-transfection was treated with and without 5 mM DTT for 1 hr. Immunoblotting with an antibody that recognizes tagged ATF6 shows both the ER-resident uncleaved and cleaved nuclear forms. Quantification of the nuclear ATF6 expressed as a percentage of the full-length form is shown below the tubulin panel.
Feng et al. BMC Cell Biology 2009 10:61 doi:10.1186/1471-2121-10-61