Ablation of PERK in 832/13 cells impairs beta cell proliferation but does not increase cell death. A. BrdU incorporation is significantly reduced in cells infected with AdDNPerk at 24 hr post-transduction cells compared to AdLacZ infected cells. The data represent two thousand cells for each treatment in two independent experiments (*p < 0.05). B. Cell Death. The percentage TUNEL-positive 832/13 cells infected with the AdDNPerk was low and was not significantly different than the cells infected with the AdLacZ control. A MOI of 20 was used for these experiments.
Feng et al. BMC Cell Biology 2009 10:61 doi:10.1186/1471-2121-10-61