Expression of AdDNPerk in 832/13 cells down-regulates insulin mRNA levels. A. Expression of DNPERK was detected by tag c-myc antibody and the eIF2α [P]/total eIF2α was reduced when two different multiplicities of infection were used. (MOI of AdLacZ1 and AdDNPerk1 = 10; MOI of AdLacZ2 and AdDNPerk2 = 20). B. At 48 hr post-transduction, the expression of insulin gene, MafA, NeuroD, and Glu2 was significantly reduced in AdDNPerk-832/13 cells. The data are expressed relative to the AdLacZ-832/13 (= 100%) ± SE, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001.
Feng et al. BMC Cell Biology 2009 10:61 doi:10.1186/1471-2121-10-61