In vitro interaction of SIRT1 and zyxin. (A) Diagram of zyxin full length (FL) and its various deletion mutants. (B) In vitro mapping of zyxin domains associates with SIRT1. HEK293T cells expressing the indicated zyxin-deletion mutant were lysed and the resulting lysates were incubated with GST-SIRT1 or control GST proteins immobilized on glutathione sepharose beads. Bound proteins were probed with anti-HA antibody. HA-zyxin or its mutants in lysates were revealed by immunoblotting with anti-HA antibody. The amounts of GST-SIRT1 and control GST proteins were revealed by amido black staining.
Fujita et al. BMC Cell Biology 2009 10:6 doi:10.1186/1471-2121-10-6