Interaction between SIRT1 and zyxin in yeast. (A) Schematic representations of SIRT1 are shown with the sirtuin homology domain. The bar below the SIRT1 structure shows the region used as the bait domain in the yeast two-hybrid system. (B) Schematic diagram of zyxin and its deletion mutants are shown with their domain structures and representative binding partners. (C) In the yeast two-hybrid binding assay, the yeast reporter line (AH109) was co-transformed with plasmids encoding the SIRT1 catalytic domain and various zyxin deletion mutants on a 4-DO (drop out) medium. The line was photographed after 14 days of incubation.
Fujita et al. BMC Cell Biology 2009 10:6 doi:10.1186/1471-2121-10-6