Additional file 1.
Phenotypic analysis of the oleate-grown Δarf1Δarf3 S. cerevisiae strain. Serial dilutions of wild-type (WT) yeast cells (strain BY4741) and yeast cells deficient in Arf1 (Δarf1), Arf3 (Δarf3), or Arf1 and Arf3 (Δarf1Δarf3) expressing EGFP-PTS1 were spotted onto plates with oleate as a sole carbon source. The plates were subsequently incubated at 30°C for five days. (A) Oleate consumption was scored by halo formation. (B) The subcellular distribution pattern of EGFP-PTS1 was visualized by fluorescence microscopy. The scale bar represents 5 μm. (C) The number of peroxisomes per cell was counted in randomly selected cells. The mean number of peroxisomes per cell is indicated by an asterisk. At least 150 oleate-grown cells were scored.
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Anthonio et al. BMC Cell Biology 2009 10:58 doi:10.1186/1471-2121-10-58