Additional file 4.
Effects of the inhibitors used in the present study on the activities of ERK1/2 in ET-1 untreated cells. The data provided represent the immunofluorescence analysis of the stability of fluorescence intensity after cells were treated with inhibitors compared with vehicle treatment. Serum-starved cells were treated with variety of inhibitors indicated or DMSO for 30 min. Phosphorylated ERK1/2 was determined by immunofluorescence with an anti-phospho-ERK1/2 antibody. The bar graph shows no significant effects of the inhibitors on phosphorylated ERK1/2 in ET-1 untreated control cells. The upper panel indicates representative images of immunofluorescence showing the phosphorylated ERK1/2 from samples treated with different inhibitors. Data represent mean ± S.E.M.
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Chen et al. BMC Cell Biology 2009 10:52 doi:10.1186/1471-2121-10-52