Additional file 3.

The Ca2+ chelator EGTA abolished thapsigargin-induced activation of ERK1/2 in ET-1 untreated starved cells. The data provided represent the immunofluorescence analysis of inhibitory effect of the Ca2+ chelator EGTA on extracellular Ca2+influx through thapsigargin-induced store-operated Ca2+ channels. Serum-starved cells were treated with 1 μM of thapsigargin with or without 5 μM of EGTA for 15 min. Phosphorylated ERK1/2 was determined by immunofluorescence with an anti-phospho-ERK1/2 antibody. The bar graph shows effect of thapsigargin on phosphorylated ERK1/2 in the presence or in the absence of EGTA. The upper panel indicates representative images of immunofluorescence showing the phosphorylated ERK1/2 from samples given the different treatments. Data represent mean ± S.E.M. *** p < 0.001 compared with the vehicle value.

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Chen et al. BMC Cell Biology 2009 10:52   doi:10.1186/1471-2121-10-52