Additional file 2.

Effect of ET-1 on activation of ERK1/2 in HASMCs in the absence of external Ca2+. The data provided represent the immunofluorescence analysis of ET-1-induced phosphorylation of ERK1/2 in the absence of external Ca2+by replacing culture medium with PBS. Serum-starved cells were placed in the presence or absence of external Ca2+ for 3 min by replacing culture medium with PBS plus 1 mM EGTA prior to addition of ET-1. Phosphorylated ERK1/2 was determined at 10 min after the addition of 10 nM of ET-1 by immunofluorescence with an anti-phospho-ERK1/2 antibody. The bar graph shows effect of ET-1 on phosphorylated ERK1/2 in the absence of extracellular Ca2+. The fluorescence intensities of phosphorylated ERK1/2 are expressed relative to the quiescent state in the presence of external Ca2+. The upper panel indicates representative images of immunofluorescence showing the phosphorylated ERK1/2 from samples given the different treatments. Data represent the mean ± S.E.M. *** p < 0.001. ns = non-significant.

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Chen et al. BMC Cell Biology 2009 10:52   doi:10.1186/1471-2121-10-52