Confocal microscopy illustration of samples of MCF-7 cells A-C) without treatment; D-F) treated 30 minutes with heparitinase (in figure F a group of three cells), Cells were stained with ErbB4 carboxy-terminus specific HFR-1 antibody. Accumulation of immunoreactive perinuclear granules is indicated by arrows. G) Activity of Heparitinase was controlled by simultaneous Western analysis of ErbB4 cleavage from parallel samples.
Määttä et al. BMC Cell Biology 2009 10:5 doi:10.1186/1471-2121-10-5