Endogenous SLMAP localises at the mitochondria and is targeted by the TA2. Cells were stained with anti-SLMAP (a) and anti-TOM20 (b) to analyze endogenous co-distribution (c). Cos7 cells were transfected with 6Myc-SLMAP1-TA1, and co-stained with anti-Tom20 (d) and anti-6Myc (e), the images were then merged (f) arrow points to an anti-Tom20 stained mitochondria. Cos7 cells transfected with 6Myc-SLMAP1-TA2 and co-stained with anti-Tom20 (g) and anti-6Myc (h) and the images were then merged (i) arrow points to anti-6Myc stained structure with no mitochondria present.
Byers et al. BMC Cell Biology 2009 10:48 doi:10.1186/1471-2121-10-48