Targeting of the SLMAP1-TA2-2L mutant. The targeting of SLAMP1-TA2 in Cos7 cells can be altered to that of SLMAP1-TA1 by increasing the hydrophobic moment of the membrane spanning section with 2 substitutions: A10/L and A11/L. Wild type GFP-tagged SLMAP1-TA2 was co-transfected with Myc-tagged SLMAP1-TA2 with the 2 substitutions (Myc-TA2-2L) and co-stained with anti-GFP (a) and anti-6Myc antibodies (b) the overlay of the two shows that there is incomplete overlap (c). In cells stained with antibodies to TOM20 (d) and transfected with Myc-TA2-2L and stained with anti-6Myc antibodies (e) there are large numbers of mitochondria which do not co-localize with any of the mutant (f). Wild type GFP-tagged SLMAP1-TA1 was co-transfected with Myc-TA2-2L and co-stained with anti-GFP (h) and anti-6Myc antibodies (i) when the two images were merged there was almost total co-localization (j).
Byers et al. BMC Cell Biology 2009 10:48 doi:10.1186/1471-2121-10-48