Sub cellular distribution of SLMAP from liver. Rat liver homogenate was fractionated by sucrose gradient centrifugation. Western blot analysis demonstrated that the cis-Golgi marker, α-mannosidase resides in the stacked Golgi fraction 1 (lane SGF1). Endogenous SLMAP (91 kDa) proteins are distributed among fractions where the ER marker calnexin is expressed (lanes B1; S3; SGF3). H (homogenate); PNS (postnuclear supernatant); SGF (stacked Golgi fraction); P (pellet).
Byers et al. BMC Cell Biology 2009 10:48 doi:10.1186/1471-2121-10-48