Additional file 3.

Annexin-V staining of irradiated p53-/- and p53+/+ ES cells. P53-/- cells and D3 cells that had been passaged twice without feeders (P2 cells) have been irradiated with 2 Gy and harvested after 24 and 48 hours. Cells were resuspended in Ca-containing buffer in the presence of Annexin V-FITC and propidium iodide and analysed by a BD FACScan Flow Cytometer. The data shown in the graph represent the relative number and standard deviation of Annexin-V positive cells of three independent experiments.

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Solozobova et al. BMC Cell Biology 2009 10:46   doi:10.1186/1471-2121-10-46