Figure 4.

Proliferation and apoptosis assays of ES cells in the presence and absence of active p53. (A) R1 cells were treated with 10 μM α-pifithrin, μ-pifithrin or carrier 24 hours after plating. Two hours after drug addition, cells were irradiated and after additional two hours, the medium was replaced with normal growth medium. After two weeks, colonies were counted. The graph shows mean values and standard deviations of three to six independent experiments. The number of colonies from non-irradiated cells was set to 100%. (B) D3 or p53-/- ES cells were irradiated with IR 24 hours after plating. After two weeks, colonies were counted. The graph shows mean values and standard deviations of three independent experiments. The number of colonies from non-irradiated cells was set to 100%. (C) P53-/- cells and D3 cells passaged once without feeders (P1 cells) were irradiated with 2 Gy and analysed for Annexin V staining after 24 and 48 hours. The graph shows the relative number and standard deviation of Annexin-V positive cells of three independent experiments. (D) D3 or p53-/- ES cells were irradiated with 2 Gray of IR and analysed at the indicated times by MTT assay. Mean values and standard deviations were calculated and plotted. The numbers for the two cell lines and conditions at day one were set to 1.

Solozobova et al. BMC Cell Biology 2009 10:46   doi:10.1186/1471-2121-10-46
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