p53 accumulates in the nucleus of irradiated ES cells. R1 (A, C) and D3 (B) stem cells, grown on feeders, were irradiated two days after plating with 7.5 Gray IR (A, B) or with 30 J/m2 UVC light (C) and harvested at the indicated times. Cells were fixed in acetone/methanol, permeabilized with Triton-X-100 and incubated with the anti-p53 antibody Pab 421. After incubation, cover slips were washed and incubated with an antibody directed against mouse IgG, coupled to Alexa-Fluor-488 (green). To visualize the nuclei, cover slips were incubated with Draq5 (blue).
Solozobova et al. BMC Cell Biology 2009 10:46 doi:10.1186/1471-2121-10-46