Bub2 overexpression causes cytokinesis and septation defects that are rescued by co-overexpression of Tem1. Cells were grown overnight (15 hours) in YPD or YPGR, then fixed. Strains used were: GAL1-BUB2 (KSY 129), GAL1-BUB2 bub2Δ (KSY130), GAL1-BUB2 bfa1Δ (KSY169), and GAL1-Bub2 GAL1-Tem1 (KSY171). Cells with (+ zym) and without zymolase treatment were examined under a microscope and the percentage of cells with chains (3 or more cell bodies) was calculated. Results shown are an average of three independent experiments, and error bars represent the standard deviation. 200 cells were counted for each treatment.
Park et al. BMC Cell Biology 2009 10:43 doi:10.1186/1471-2121-10-43