Expression of Endo180 protein and mRNA by four established HSC lines from three different species. Detection of Endo180 and β-actin proteins (A) and mRNAs (B) was performed as described in Figure 2. Results are representative of three (A) or two (B) experiments. (C) Uptake and degradation of denatured collagen by different HSC lines. Cells were grown in 6-well plates, washed, pre-incubation for 45 min at 37°C, and then incubated for 2 h at 37°C with 10 μg/ml 125I-TC-collagen. Experimental procedure is as in the legend to Figure 3. (D) The calculated percentage of internalized 125I-TC-collagen degraded by different HSC lines. Results are shown as means ± SD for triplicate wells and are representative for at least two similar experiments. Cells used from a new passage was treated as a separate experiment. Data are normalized for cell number.
Mousavi et al. BMC Cell Biology 2009 10:39 doi:10.1186/1471-2121-10-39