Analysis of expression of TLRs on P815 cells. (A) For RT-PCR analysis, the gene products were separated in a 1.5% agarose gel. Lane 1–5 represented DNA marker, β-actin (288 bp), TLR3 (240 bp), TLR7 (262 bp) and TLR9 (297 bp), respectively. (B) For flow cytometry analysis, cells were incubated with FITC-conjugated goat anti-mouse TLR9 monoclonal antibody or incubated with rabbit anti-mouse TLR3, TLR7 polyclonal antibodies at 37°C for 1 h. (C) For immunofluorescent microscopy, cells were treated with the above-mentioned procedures and analyzed with a laser scanning confocal microscope.
Yang et al. BMC Cell Biology 2009 10:37 doi:10.1186/1471-2121-10-37