Table 1

Primers employed in RT-PCR

Primer

T_m

product

Accession-No.

5'-CAGTGAATTCATGGCCGGT TGGCAGAG-3'

5'-CGATGGATCCAGCAGCTAGAACCCAGAGTC-3'

68°C

71°C

446 bp

ORF

(bp 99 → 526)

huProfilin2a

NM_053024

5'-TTGAATTCATGAGTGACTGGAAGGGCTACA-3'

5'-TTGGATCCGTTCACGGTTTATTCTGGTCTCC-3'

65°C

68°C

467 bp

ORF

(bp 59 → 524)

mProfilin3 NM_029303

5'-CAGTGAATTCAGCATGAGTCACTTGCG-3'

5'-GATGGATCCTTAGTTTCCCTTTTTCCTTAG-3'

63°C

64°C

405 bp

ORF

(bp 861 → 1250)

mProfilin 4

NM_028376

5'-AGTGAATTCGGGAACATGAGCCATT-3'

5'-GATGGATCCCTCTGATGACTTAACTTCCT-3'

61°C

65°C

406 bp

ORF (bp171 → 557)

huProfilin4

BC029523

5'-TAGAATTCATGGATGGATGATATCGCCGCGC-3'

5'-AAGGATCCAAGCCATGCCAATCTCA-3'

67°C

70°C

1236 bp

ORF (bp74 → 1282)

hu β-actin, NM_001101

5'-CAGTGAATTCTTTACCGACCACCAAGAAACTCAG-3'

5'-CGATGGATCCTCATATGGCACCCGAAAAGAGC-3'

68°C

71°C

668 bp, FH1 + part. FH2-domain (bp904 → 1551)

huDiaphanous3 NM_030932

Behnen et al. BMC Cell Biology 2009 10:34   doi:10.1186/1471-2121-10-34