Additional File 1.

Immunolocalization of PFN4-related protein in spermatids and testicular spermatozoa isolated from human testis. A1-A3) and B1-B3) show dual labelling and confocal microscopy of human round and elongating spermatids employing indirect PFN4 immunofluorescence (green) and PNA lectin binding (red); nuclei were stained with DAPI (dark blue). A4 and B4 show corresponding phase contrast image. Note PFN4 immunofluorescence in acroplaxome and manchette (high lightened by yellow arrows); spermatocyte shows weak cytoplasmic staining. Scale bars correspond to 5 μm. C1–C3 and D1–D3 show dual labelling and confocal microscopy of human testicular spermatozoa employing indirect PFN4 immunofluorescence (green) and PNA lectin binding (red); nuclei were stained with DAPI (dark blue). C4 and D4 show corresponding phase contrast image. Scale bars correspond to 20 μm and 5 μm, respectively.

Format: PDF Size: 778KB Download file

This file can be viewed with: Adobe Acrobat Reader

Behnen et al. BMC Cell Biology 2009 10:34   doi:10.1186/1471-2121-10-34